Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes

Background Sf-9 cells, derived from Spodoptera frugiperda, are widely used for recombinant protein production using the baculovirus expression vector system (BEVS). However, this results in a productive viral infection and cell lysis. Therefore, a non-lytic, plasmid-based expression system for suspension Sf-9 cells would be a valuable alternative to the BEVS for rapid, scalable, and highyielding recombinant protein production and for the generation of stable Sf-9 cell lines [4]. In this work, we present a simple, efficient and cost-effective plasmidbased method for transient expression of recombinant proteins in Sf-9 cells cultivated in serum-free suspension mode in a high-throughput culture system, TubeSpin bioreactor 50 tubes (TubeSpins).